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. 2009 Jul 16;66(17):2933–2942. doi: 10.1007/s00018-009-0082-7

Fig. 3.

Fig. 3

The 5′-extended long form of MOP is a short-lived protein. a Schematics of the constructs used. The initiation codon (dotted line) indicates a point mutation of ATG to ACG. b Time course of protein degradation. The relative remaining LUC activity in NMB cells transfected transiently with the constructs shown in a was determined by incubating the cells in serum-free medium at 37°C for 0–18 h. Cell lysates were assayed for luciferase reporter activity, expressed as the ratio LUC/β-gal. Data represent the means and standard errors of three independent assays conducted in duplicate. t 1/2 = half-life