Fig. 3.

Effect of ARNT on EGF-induced 12(S)-lipoxygenase gene expression. a Cells were transfected with 20 nM ARNT siRNA by lipofection. After EGF treatment for 18 h, total RNA was extracted for reverse transcription PCR with 12(S)-lipoxygenase (12(S)-LOX), ARNT and GAPDH primers. b Quantitative real-time PCR analysis of 12(S)-lipoxygenase was performed as described under “Materials and methods”. Each column represents the mean ± SEM (n = 3). c Cells were transfected with pXLO-7-1 and various amounts of ARNT siRNA or scramble oligonucleotides (SC) by lipofection. After EGF treatment for 18 h, the luciferase activities and protein concentrations were then determined and normalized. Values represent means ± SEM of three determinations. Expressions of ARNT and β-actin proteins were analyzed by western blot analysis using anti-ARNT and anti-β-actin antibodies, respectively