Fig. 4.

Cooperation between ARNT and c-Jun on promoter activation of 12(S)-lipoxygenase gene. a Cells were transfected with pXLO-7-1, myc-ARNT, c-Jun siRNA and scramble oligonucleotides (SC) by lipofection. The luciferase activities and protein concentrations were then determined and normalized. Values represent means ± SEM of three determinations. Expressions of myc-ARNT and c-Jun proteins were analyzed by western blot analysis using anti-ARNT and anti-c-Jun antibodies. b Cells were transfected with various amounts of ARNT siRNA oligonucleotides, pXLO-7-1 and myc-c-Jun by lipofection. The luciferase activities and protein concentrations were then determined and normalized. Values represent means ± SEM of three determinations. Expressions of myc-c-Jun, ARNT and β-actin proteins were analyzed by western blot analysis using anti-c-Jun and anti-ARNT antibodies