Fig. 8.

Restoration of antiangiogenic activity of 3-methylcholanthrene (3MC) in vivo by resveratrol or alpha-NF using Transwell and Matrigel plug assays. a Elimination of 3MC-mediated inhibition in cell motility by Y27632 and aryl-hydrocarbon receptor (AhR) antagonists. The migration of human umbilical vascular endothelial cells (HUVECs) was evaluated in microchemotaxis chambers. Cells grown in the 24-well microchemotaxis chamber were treated with the indicated chemicals and assayed at 16 h of the treatment. Numbers of cells migrating through to the lower surface of each membrane were counted, as described in “Materials and methods”. Cells migrating in the transwells were photographed and A–E represent the control, 3MC alone, 3MC + Y27632, 3MC + resveratrol, and 3MC + alpha-NF groups, respectively. Magnification ×100. b Angiogenesis was assessed using a Matrigel angiogenesis assay. The mean hemoglobin concentration of plugs excised from animals with various treatments was quantified as described in “Materials and methods”. Six samples were analyzed in each group, and values represent the mean ± SD. *Significantly different (P < 0.05 vs the control), ^#significantly different (P < 0.05 vs 3MC alone)