Fig. 4.

MALDI-MS/MS and MALDI-IMS-MS/MS whole body imaging of rats dosed at 6 mg/kg IV with vinblastine. Distribution of the precursor ion m/z 811.4 (vinblastine) and production m/z 751 (vinblastine dissociation product) is shown. Comparison of a m/z 811 and b m/z 811-751 images obtained using MALDI-IMS-MS/MS and conventional MALDI-MS/MS clearly demonstrate the advantages of ion mobility separation within MALDI xenobiotic imaging. The main difference observed is indicated; the distribution of the ions of interest within the renal pelvis. White contrast indicates vinblastine concentration. c Whole body autoradiograph showing the distribution of ³H in a 1-h post-dose rat dosed with 6 mg/kg IV ³H vinblastine, confirming the distribution observed with MALDI-IMS-MS/MS. Reprinted with the authorization of the American Chemical Society