Fig. 2.

RP-HPLC profiles of the anti-Micrococcus luteus active materials obtained from the previous purification step (size exclusion HPLC) at 220 nm. a Fraction 13, b fraction 14 of Fig. 1. An elution gradient of solvents from 5 to 70% acetonitrile/water/0.1% TFA was applied for 60 min at a flow rate of 1 mL/min. Arrows indicate the anti-M. luteus active peaks containing lucifensin. MS analysis (molecular mass 13,824 Da) and the partial N-terminal Edman degradation of the material isolated as the most prominent peak (peak 12) eluted at 33 min indicate that this may be the insect lysozyme. However, this material was inactive against M. luteus under the tested conditions. Inset Anti-M. luteus activity (clear zones in the drop diffusion test) of selected peaks delineated in the profile