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. 2009 Nov 18;67(3):455–466. doi: 10.1007/s00018-009-0194-0

Fig. 3.

Fig. 3

RP-HPLC re-purification of the combined anti-Micrococcus luteus active fractions obtained from the previous purification step (RP-HPLC, Fig. 2, arrows). An elution gradient of solvents from 5 to 70% acetonitrile/water/0.1% TFA was applied for 60 min at a flow rate of 1 mL/min. Detection at 220 nm