Fig. 5.

RP-HPLC profiles of the lyophilised retenates obtained by ultrafiltration of crude extracts from three different larval tissues at 220 nm. a Salivary glands, b fat body, c haemolymph. An elution gradient of solvents from 5 to 70% acetonitrile/water/0.1% TFA was applied for 60 min at a flow rate of 1 mL/min. Arrows indicate the anti-Micrococcus luteus active peaks containing lucifensin. Inset Anti-M. luteus activity (clear zones in the drop diffusion test) of the fractions containing lucifensin and other surrounding fractions delineated in the profile. The other peaks not numbered in the chart were collected as larger fractions at consecutive time intervals. Those fractions did not show any anti-M. luteus activity