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RP-HPLC profile of the lyophilised retenate obtained by ultrafiltration of the larval ES at 220 nm. An elution gradient of solvents from 5 to 70% acetonitrile/water/0.1% TFA was applied for 60 min at a flow rate of 1 mL/min. Arrows indicate the anti-Micrococcus luteus active peak containing lucifensin. Inset Anti-M. luteus activity (clear zones in the drop diffusion test) of individual fractions and selected peaks delineated in the profile
