Table 2.

Effects of various mutations on human prosomatostatin processing in transfected Neuro2A cells [89]

Mutants	Amino acid sequences															S-14 (%)	S-28 (%)	R	proSom (%)
	−15						−9				−5			−2	−1
WT	R	S	A	N	S	N	P	A	M	A	P	R	E	R	K	40	41	1.0	19
[A−5]	R	S	A	N	S	N	P	A	M	A	A	R	E	R	K	4	30	7.5	66
[G−5]	R	S	A	N	S	N	P	A	M	A	G	R	E	R	K	64	34	0.5	2
[S−5–N−3]	R	S	A	N	S	N	P	A	M	A	S	S	N	R	K	51	22	0.4	27
[Y−5–G−3]	R	S	A	N	S	N	P	A	M	A	Y	K	G	R	K	50	18	0.4	32
Δ[PP]	R	S	A	N	S	N	X	A	M	A	X	R	E	R	K	5	30	6.0	65
[P−9,P−6]	R	S	A	N	S	N	P	A	M	P	A	R	E	R	K	19	34	1.8	47
[P−6,P−5]	R	S	A	N	S	N	A	M	A	P	P	R	E	R	K	48	24	0.5	28
Δ[AMA]	R	S	A	N	S	N	P	X	X	X	P	R	E	R	K	55	27	0.5	18
[A−9]	R	S	A	N	S	N	A	A	M	A	P	R	E	R	K	51	4	0.1	44
[A−5]	R	S	A	N	S	N	P	A	M	A	A	R	E	R	K	4	30	7.5	66
[A−9,A−5]	R	S	A	N	S	N	A	A	M	A	A	R	E	R	K	5	25	5.0	70
Δ[PAMAP]	R	S	A	N	S	N	X	X	X	X	X	R	E	R	K	13	56	4.3	31

The bold values correspond to values obtained for the peptides in which a sequence or a residue was modified or deleted

The amounts of S-28, S-14 and precursor were evaluated by RIA using antibodies against somatostatin-14

R represents the ratio S-28/S-14