Fig. 9.

a Selected GsnVHHs protect against gelsolin proteolysis. SDS-PAGE Coomassie stained gels showing proteolysis of gelsolin over time (2, 5, and 8 days) in the presence of 100 μM Ca²⁺. GsnVHHs 2, 4, 6, and 11 accelerate gelsolin degradation in comparison to the control (c, control). GsnVHH 3 and 9 slow down degradation (asterisks). Experiments were carried out at 16°C. b Crystal structures of GsnVHHs 3, 9, and 13 are superpositioned. The third loop region differs among the three VHH molecules. The loop region of GsnVHH 13 is shown in blue, the one of GsnVHH 3 is shown in red and the one of GsnVHH 9 is shown in yellow