Table 2.

Thermodynamic parameters of the interaction between GsnVHHs and gelsolin with or without calcium, determined by isothermal titration calorimetry (ITC)

	GsnVHH 3		GsnVHH 9		GsnVHH 11		GsnVHH 13
	−Ca	+Ca	−Ca	+Ca	−Ca	+Ca	−Ca	+Ca
N	No significant binding	0.860 ± 0.003	0.900 ± 0.009	1.000 ± 0.009	0.900 ± 0.003	0.900 ± 0.003	No significant binding	0.950 ± 0.004
∆H (kcal/mol)		−17.60 ± 0.98	−11.70 ± 1.66	−17.20 ± 2.07	−12.50 ± 0.75	−13.20 ± 0.84		−6.97 ± 0.51
T∆S (kcal/mol) with T = 303.15 K		−7.61	−1.80	−7.85	−0.83	−2.06		4.18
∆G (kcal/mol) ∆G = ∆H−T∆S		−9.89	−9.88	−9.35	−11.67	−11.14		−11.15
Kd (M)		(6.54 ± 0.39) E-8	(7.52 ± 1.10) E-8	(1.79 ± 0.02) E-7	(3.65 ± 0.54) E-9	(1.00 ± 0.11) E-9		(9.26 ± 1.61) E-9