Fig. 6.

Effect of geldanamycin (GA) on the Thr308 phosphorylation of w.t. and Ser129Ala mutant Akt. HEK 293T cells were transfected with w.t. Akt or Ser129Ala Akt mutant for 24 h and treated with 10 μM GA for the last 1 h of transfection, where indicated; 10 μg of total proteins from cell lysates were analyzed by Western blot with the indicated antibodies. In the lower panel, quantification of phospho-Thr308 and phospho-Ser129 of three different experiments (means  ± SEM) are shown