Fig. 7.

Cellular calcium perturbation increased nuclear accumulation of Smad3. a 293T cells transfected with Flag-Smad3 or EGFP-Smad2 were treated with A23187 (2 μM) or TG (1 μM) for 24 h, followed by western blot analysis of the nuclear and cytoplasmic extracts. b 293T cells were transfected with Flag-Smad3 or EGFP-Smad2, treated with BAPTA–AM (10 μM) for 45 min before apoptosis was induced. Western blot was performed on nuclear and cytoplasmic extracts. Histone-3 and α-tublin were used as markers of nuclear and cytoplasmic extracts, respectively