Fig. 7.

Progesterone inhibits calcium signals to thapsigargin and vice versa. a Fura-2-loaded HEK293 or PHM1-31 cells were treated with 160 μM progesterone (P4) (+) or ethanol (−) at 37°C for 20 min. Then, calcium responses to 1 μM thapsigargin were analyzed. b Fura-2-loaded HEK293 or PHM1-31 cells were treated with 1 μM thapsigargin or ethanol (control) at 37°C. After 3 min, calcium responses to 160 μM progesterone were analyzed. The data represent means ± SD (n = 3). **p < 0.01, ***p < 0.001 (treatment vs. control)