Fig. 5.

Effect of IL-1Ra upregulation by GW501516 on the proliferation and migration of VSMCs. a Cells transfected with an IL-1Ra siRNA for 24 h were pretreated with GW501516 and/or a neutralizing anti-TGF-β antibody for 24 h and then incubated with IL-1β for 24 h in DMEM containing 5% FBS. VSMC proliferation was determined by MTT assay (n = 4). b,c Serum-starved cells were transfected with an IL-1Ra siRNA for 24 h, and then pretreated with GW501516 and/or a neutralizing anti-TGF-β antibody for 24 h. Cell monolayers were scraped with a razor blade, and then the cells were incubated with IL-1β in fresh medium containing 5% FBS. After incubation for 96 h, the number of cells that migrated across the wound was counted. Representative photographs (b) and cell number (c) are shown as the mean ± SE (n = 4). *P < 0.01 versus 5% serum-treated group; ^# P < 0.01 versus IL-1β-treated group; ^† P < 0.01, ^‡ P < 0.01 versus IL-1β + GW501516-treated group