Fig. 6.

ICOSIg activates p38-MAPK signal pathway in DCs. The 5-day immature DCs derived from wild or ICOSL^−/− mice were resuspended in 100 μl 1640 culture medium containing 1% FCS, with 5 × 10⁶ total cell numbers in each group. After being incubated for different times (a) or 60 min (c) with 200 μg/ml ICOSIg or IgG (a,b), or with 100 ng/ml LPS (c), DCs were collected and treated with cell lysis solution; the lysate were then used for detecting phosphorylated and total p38 protein using western blot. The relative gray scale values of each p-p38 MAPK band were also listed (b vs a and d vs c), which were obtained by Smartview Software and calibrated with those of each β-actin, respectively. p-p38 Phosphorylation p38 protein, t-p38 total p38 protein, β-actin inner reference protein, W- wild-derived DCs, K- ICOSL^−/−-derived DCs (data are one representative of three)