Fig. 8.

DCs pretreated by ICOSIg induces DTH. Immature DCs derived from BALB/c mice were pulsed with P815AB and tt peptides after being treated with IgG1 or ICOS-Ig (60 μg/ml) in vitro, with or without anti-IL-6 treatment. In one group, DCs were first blocked with anti-ICOSL for 1 h before being treated as described above. The priming ability of the conditioned, peptide-pulsed DCs was examined by transfer into homogenic recipient mice, which were assayed at 2 weeks for skin test reactivity to the eliciting peptide. Reactivity was measured as the increase in footpad weight of peptide-injected footpads over that of vehicle-injected counterparts. Untreated Normal mouse with no treatment. Each of the results (mean ± SD) is from one experiment representative of three, with at least five mice per group per experiment (*P < 0.01)