Fig. 7.

CPZ treatment attenuated colocalization of granzyme B and LAMP-1 in NK cells stimulated by PEC cells. NK cells with CPZ pretreatment (+CPZ) or non-treatment (−CPZ) were stimulated by PEC for 0 or 60 min. The following immunostaining was the same as in the Fig. 5 legend. Anti-LAMP-1 antibody was diluted in 1:150. LAMP-1 is shown in green. Granzyme B (GrmB) is shown in red. Nucleus is shown in blue. For leupeptin treatment assay, all cells were pre-cultured for 16 h in the presence or absence of leupeptin (15 µM). Scale bar is 10 µm. Data are representative of three independent experiments