Fig. 8.

CHC RNAi treatment attenuated colocalization of granzyme B and LAMP-1 in NK cells stimulated by PEC cells. After NK cells with CHC-specific RNAi pretreatment (CHC) or scrambled RNAi (scrambled) pretreatment were stimulated by PEC for 0 or 60 min, colocalization of LAMP-1 and granzyme B was observed by immunostaining. Anti-LAMP-1 antibody was diluted in 1:150 and anti-granzyme B was diluted in 1:200. LAMP-1 is shown in green. Granzyme B (GrmB) is shown in red. Nucleus is shown in blue. Leupeptin treatment is the same as in Fig. 7