Quantity of granzyme B in the supernatants and NK92 cells. 1 × 106 CPZ pretreated or non-pretreated NK92 cells were stimulated with 1 × 105 PEC (a–c) or K562 cells (d–f) for 1, 3, and 5 h, respectively. The supernatants were collected to test the granzyme B with ELISA (a, d). NK92 cells were harvested to detect the granzyme B by Western blot (b, e). c, f Density ratio of granzyme B versus actin band. Data are representative of three independent experiments