Expression of VDR responsive genes was studied at m-RNA levels using Q-PCR. Fold change expression values of VDR responsive genes, relatively compared between DG75-EBNA5 and DG75-EBNA3, untreated and treated with 1,25(OH)2D3. VDR responsive genes (except C-FOS) were upregulated in 1,25(OH)2D3 treated DG75-EBNA5 compared to untreated DG75-EBNA5. Notice that 1,25(OH)2D3 treatment did not result in VDR-responsive gene induction in DG75-EBNA-3 cells