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Association of GDH- and AIF-FLAG constructs with mitochondria. HeLa S3 cells were transiently transfected with vectors encoding GDH and AIF as FLAG tagged proteins. After 24 h cells were stained with MitoTracker (MT) and subjected to FLAG-immunocytochemistry. The fluorescence micrographs show nuclei (DAPI), expressed recombinant proteins (FLAG), and mitochondria (MT). Bar 10 μm
