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ChIP assay of tnf-α promoter occupation in taurocholate-induced AP in rats. a Putative binding sites for transcriptional factors in the tnf-α promoter analyzed by MatInspector Software. b The immunoprecipitated samples with indicated antibodies were analyzed by PCR using primers of the tnf-α promoter region. c ImageJ analysis of PCR signals. α-Actin gene has also been included as a negative control of the ChIP experiment
