Fig. 6.

HIP1 colocalizes with transferrin receptor (TfR). a A region of a cell coexpressing tdTomato-HIP1 (red) and TfR-pHl (green) was visualized in TIRF, under two pH conditions (left, pH 7.4; right, pH 5.5). Both images are filtered under the same conditions, but scaled differently, so that the low-intensity internalized TfR-pHl vesicles would be visible. b Colocalization of HIP1 and TfR assemblies was measured under the two pH conditions (mean ± SEM, regions from seven cells, P < 0.005, paired t-test)