Fig. 5.

HDAC3 antagonizes the CXCR4-dependent migration of metastatic breast cancer cells. a Both depletion of CREB3 and overexpression of HDAC3 suppress cell migration. MDA-MB-231 cells were transfected with either siRNAs or plasmids before seeding into the upper chamber. The data presented are the means ±SD of at least three independent experiments. ^# P < 0.05 versus control. b HDAC3 antagonizes the CCL12-induced migration of MDA-MB-231 cells. Two days after transfection, the cells were scratched with a 200-μl pipette tip. Floating cells were washed with DMEM. The cells were photographed under a microscope 12 h after scratching. CXCL12 was used as a positive control for invasive latency. The data presented are the means ±SD of at least three independent experiments. ^# P < 0.05 versus control. c Both the depletion of HDAC3 and overexpression of CREB3 enhance cell adhesion. Adhesion assays were done as described in “Materials and methods”