Fig. 2.

Bcl-2 family members can perturb mitochondrial fission and fusion dynamics. HeLa cells were transfected either with vector (pcDNA3.neo), Flag-Bcl-xL, or Flag-Bcl-2 encoding plasmids, in combination with pDsRed-Mito reporter plasmid to stain mitochondrial networks. Cells were fixed 18 h later and immunostained for Flag to reveal Bcl-xL or Bcl-2 localization patterns. As compared to vector-transfected cells, note that Bcl-xL-transfected cells displayed fragmented mitochondrial networks, whereas Bcl-2-transfected cells contained mitochondrial networks that were highly fused