Fig. 5.

Wnt5a activates PDE6 through the FZ2 and transducin cascade. a PDE6 activity was measured in melanoma cell lines with different pattern of PDE6 expression and in HaCaT cell line incubated with or without 20 ng/ml Wnt5a for 1 h. b PDE6 activity after stimulation with Wnt5a was measured in Ma-Mel-04 and Ma-Mel-12 melanoma cells pretreated with 50 ng/μl antibody against FZ2 for 30 min. c PDE6 activity after stimulation with Wnt5a was measured in melanoma cells cultivated with 50 ng/ml of pertussis toxin for 24 h. d PDE6 activity in untrasfected and transfected with shRNA coding plasmids for PDE6-γ, Ma-Mel-04 with and without additional stimulation/inhibition with Wnt5a or PT. Statistically significant differences (P < 0.05) are marked with an asterisk