Table 1.
Similar shapes of murine occludin406–521 in its free sulfhydryl (Occl-Cys) and sulfhydryl-masked form (Occl-Cys-R) are shown by similar ratios of R S: R S,calc
| M app (g/mol) | R S (nm) | R S,calc (nm) | R S/R S,calc | |
|---|---|---|---|---|
| Occl-Cys (1.16 mg/ml) | 23,100 ± 2,500 | 2.91 ± 0.03 | 2.27 | 1.28 |
| Occl-Cys-R (0.75 mg/ml) | 14,800 ± 1,500 | 2.49 ± 0.03 | 1.92 | 1.29 |
Apparent molecular mass (M app.) and Stokes radius (R S) were determined by static and dynamic light scattering, respectively
R S,calc. was calculated from the molecular mass assuming a globular molecule
A systematic error in M app. of about 10% was taken into consideration because of possible uncertainties in the refractive index increment and protein concentration