Fig. 3.

HCVne particles require low pH for internalization. A Schematic diagram representation of the experiment. B Huh7 cells untreated (a) or incubated with 5 mM NH₄Cl (b), 20 μM chloroquine (c), or 50 nM bafilomycin (d) were added with particles as described in (A) and immunolabeled with anti-core (green) and anti-EEA1 (red) antibodies. Inset images show a higher magnification of the boxed section of the merged pictures. Bars a,b,d 16 μm, c 20 μm. C Image-Pro quantification of colocalization. The control was arbitrarily set at 100% and all other values are a percentage of this. D Quantification of colocalization from cells treated with E64 (50 μm, 2.5 h) and pepstatin A (50 μm, 2.5 h). Cells were pretreated with the inhibitors as described in (A). ***P < 0.001