Fig. 4.

HCVne particles are internalised via a clathrin-dependent pathway. A HCVne particles and Alexa 546-transferrin (red) in Huh7 cells, processed for immunofluorescence with anti-core (green) and anti-EEA1 (blue) antibodies. Arrows show individual endosomes positive for the two markers (insets). Bar 16 μm. B Schematic diagram representation of the experiment. C Quantification of colocalization of HCVne particles and transferrin with EEA1. Huh7 cells pre-treated with increasing concentrations of sucrose. Following addition of HCVne particles or transferrin, cells were fixed and immunostained either with anti-core/anti-EEA1 or anti-EEA1. **P < 0.01, ***P < 0.001