Fig. 6.

Implication of host cell proteins in HCVne trafficking. A,D Schematic representation of the experiment. HepG2 cells were pre-treated with sodium orthovanadate (5 mM, 30 min), okadaic acid (0.5 μM, 30 min), UO126 (1 μM, 1.5 h), PD98059 (5 mM, 1.5 h), and SB202190 (20 μM, 1.5 h). Particles were added for either 15 min (B) or for 20 min, removed and incubated until 80 min (E). Cells were fixed, immunostained with anti-core (green) and anti-EEA1 (red) and observed with confocal microscopy. Then, 9–15 confocal sections from three to five different experiments were selected and quantified with Image-Pro Plus software. C HCVne particles were added in HepG2 cells for indicated times. EGF (50 nM) and anisomycin labeled (A, 10 μM) were used as positive controls. Lysates were analysed by western blotting with anti-phospho-p38 (a) or anti-p38 (b) antibodies. Densitometric analysis is expressed in arbitrary units (c). *P < 0.05, **P < 0.01, ***P < 0.001