Fig. 6.

Overexpression of MAT1A repressed HCC cell migration and invasion. a MAT1A protein expression levels in two J7 cell lines overexpressing MAT1A (clones #11 and #13) or in the J7-vector control cell lines (clones #5 and #6). b Zymography assayed for MMP activities. J7 cells and MAT1A overexpressing sublines (4 × 10⁶) were plated in DMEM medium. c Invasion or d migration of two MAT1A overexpression cell lines and two control cell lines. Cells were added to the upper chamber of Transwell units and incubated for 24 h, as described in Fig. 5, and repression of invasion or migration was quantified as fold changes using J7-vector as a control. All assays were repeated at least three times. Differences were examined using Student’s t-test. **p < 0.01. Significance was assessed by comparison with the control vector