Fig. 1.

hINO80 knockdown results in slow cell growth. a Whole-cell lysates of parental HeLa-S3, vector, si-hINO80-2 and si-hINO80-3 cells were analyzed for the expression of hINO80 by immunoblottings. The expression of actin was also analyzed as loading control. b The growth rates of parent, vector, and si-hINO80-2/3 cells were determined by counting viable cells using the Tryphan Blue exclusion method. The data are presented as mean ± standard deviation (SD) from three separate experiments. c Indicated cells were transfected with control or synthetic hINO80-specific siRNAs for 24 h, and further cultured for 48 h. Live and dead cells were determined by the Tryphan Blue exclusion method