Fig. 9.

hINO80 is critical for the maintenance of chromosome integrity. a Twenty metaphase spreads for each of the indicated cells were subjected to karyotype analysis. By setting the karyotype of vector cells (all identical in 20 spreads) as a background, the chromosomal aberrations that were additionally generated in si-hINO80-2 cells were evaluated. The frequency of various types of chromosomal aberrations was depicted as a graph. Abbreviations are as follows: loss chromosome loss, gain chromosome gain, trans translocation, del deletion, tas telomeric association, dic dicentromeric chromosomes, chrb chromosome breaks, chtb chromatid break, mar marker chromosomes. b Representative images of the karyotypes analyzed in (a) are shown. Symbols are same as in (a) and as follows: plus (+) sign chromosome gain, minus sign (−) chromosome loss, t translocation. An open arrow head indicates the chromosomal aberrations already present in control cells and the closed arrow head indicates those newly generated in h-pSuper-hINO80 cells. c HCT116 cells were transfected with either non-specific control siRNA or hINO80-specific siRNA. Aliquots of transfected cells were analyzed for the expression of hINO80 and actin (loading control) by immunoblottings. d The remaining cells were subjected to karyotype analysis. The frequency of various types of chromosomal aberrations detected from 20 mitotic spreads was depicted as a graph. e Representative images of the karyotypes analyzed in (d) are shown. The cells transfected with control siRNA show the karyotype identical to the typical one of parental cells, which only has translocations in chromosomes 10, 16, and 18, with approximately half of the cell population missing Y chromosome. Abbreviations and symbols are same as in (a) and (b)