Fig. 2.
Schematic representations of the retroviral vector and the dsRNA hairpin. a The pLNHX vector containing the MMLV long terminal repeats (LTR), the packaging signal (Ψ), and a neomycin selection marker (neor), modified to include the S. mansoni actin 1.1 gene promoter (Act 1.1) driving the transcription of inverted regions of the S. mansoni cathepsin B1 encoding sequence linked by a spacer (loop). b RNA hairpin structure of the transcribed sequence. For clarity, the central part of the molecule has been cut away. The structure was generated using mfold (http://mfold.burnet.edu.au/)