Table 1.
The sequences of PCR primers used in this study
| Gene name | Primer sequence 5′-3′ | Product size, bp | |
|---|---|---|---|
| CK18 | External senses primer | TGCCGCCGATGACTTTAG | 454 |
| External antisense primer | CCTCTGCCCGAGTTTGTG | ||
| Internal sense primer | TCCGCAAGGTGGTAGATGAC | ||
| Internal antisense primer | CTCCATCTGTGCTTGTATCG | ||
| CD19 | External senses primer | AAGGAAGCGAATGACTGACCC | 344 |
| External antisense primer | GGAAGTCCATCATCCTGCCA | ||
| Internal sense primer | GGAAGCGAATGACTGACC | ||
| Internal antisense primer | GGGGTTCTCATAGCCACTC | ||
| CD20 | The same sense primer | TTCAAACTTCCAAGCCGTATG | 241 |
| External antisense primer | GAGTTTAAGGAGCGATCTC | ||
| Internal antisense primer | GACAGCAGAACCACATTAGAT | ||
| CD45 | The same sense primer | GCACACCAAAAGAAAAGGCTAATA | 277 |
| External antisense primer | CCATTGACATAGGCAAGTAGG | ||
| Internal antisense primer | GGAATCCCCAAATCTGCTGC | ||
| CD138 | External senses primer | ACCAGCAGCAACACCGAGAC | 606 |
| Internal sense primer | TTCACTGCTCGGGACAAGG | ||
| The same antisense primer | TCCCCATCAGGCGTAGAAC | ||
| IGHG1 constant | External senses primer | CCTGGTCAAGGGCTATTTCC | 381 |
| Internal sense primer | TCTACACTCTGAGCAGCTCAG | ||
| The same antisense primer | GGTGCATGATGGGAAGTTCAC | ||
| IGHG1 variable | External senses primer | AGGTCCAGCTQCTCGAGTCWGG (Q = G/T; W = A/T) | 394 |
| Internal sense primer | CGGTACCAAGAASAMCCTGTWCCTGCAAATGASC (S = C/G; M = A/C; W = A/T) | ||
| The same antisense primer | TCTTGTCCACCTTGGTGCTGCTG | ||
| IGHG1 germline transcript | External senses primer | AGCTGCAAGAAGAGGCCAT | 429 |
| Internal sense primer | GGCCCTTCCAGATCTTTGAG | ||
| The same antisense primer | GGATCCAGAGTTCCAGGTCACT | ||
| Igκ variable | The same sense primer | GACATTCAGCTGACCCAGTCTCCA | 311 |
| External antisense primer | GTCCTGATCAGTCCAACTGTTCA | ||
| Internal antisense primer | AGNTTGGTTCCACCRCCGAACG (N = C/T; R = T/C) | ||
| AID | The same sense primer | AGGGAGTCAAGAAAGTCACGC | 347 |
| External antisense primer | GCCTTGCGGTCTTCACAG | ||
| Internal antisense primer | GCTGAGGTTAGGGTTCCATC | ||
| RAG1 | The same sense primer | CCAAGCTGCAGACATTCTAGCACTC | 379 |
| External antisense primer | CTGGATCCGGAAAATCCTGGCAATG | ||
| Internal antisense primer | GCCTTGGCTTGGTGAACTGCTT | ||
| RAG2 | The same sense primer | CACATCCACAAGCAGGAAGTACAC | 330 |
| External antisense primer | GGTTCAGGGACATCTCCTACTAAG | ||
| Internal antisense primer | GCTTGTCAGAGTCTATGCTGCCTTTGT | ||