Table 3.
Association constants K a (mM−1) of wild-type and mutant rA2PIC peptides to various kringle constructs of Pgn
| Ligand | rK1 | K1–3 | K4 | rK4–5 |
|---|---|---|---|---|
| 6-AHA | 75 ± 11a | 107 ± 11 | 46 ± 4 | ~52 |
| Wild type | 77 ± 4 | 176 ± 30 | 34 ± 2 | 18 ± 1 |
| K406A | 42 ± 6 | |||
| K415A | 44 ± 6 | |||
| K422A | 177 ± 22 | 33 ± 3 | ||
| K429A | 170 ± 9 | 33 ± 3 | ||
| K436A | 70 ± 1 | 26 ± 10 | 32 ± 2 | 16 ± 2 |
| K452S | 8 ± 0.5 | 17 ± 4 | 11 ± 2 | 1.4 ± 0.5 |
| K452A | 11.6 ± 0.5a | 9.1 ± 0.2a | ||
| K429/36A | 17 ± 6 | 29 ± 0.5 | 16 ± 5 | |
| K429/52A | 8 ± 0.5 | 30 ± 5 | ||
| K422/29/36A | 22 ± 7 | 28 ± 1 | 18 ± 5 | |
| K415/22/29/36A | 12 ± 2 | 24 ± 1 | ~17 | |
| K406/15/22/29/36A | 53 ± 7 | 46 ± 12 | 23 ± 2 | 17 ± 4 |
| K406/15/22/29/36/52A | ~3 | ~4 | ~3 | |
| A2PIC(E442-K452/Y445[SO4]) | 29 ± 4 | |||
| A2PIC(E442-K452) | 24 ± 4 |
aFrom [18]