Fig. 5.

Mutation analysis of Intron 1 promoter-like activity. N2A cells were transfected with pGL3-Basic carrying the Exons fragment of Prnp or a series of mutants. The diagram on the right shows the Exons construct and the different mutants used. Luciferase reporter assays were performed and values were compared to those of cells transfected with the vector only. The data is presented as relative luciferase unit. Shown are the mean and SE for at least three experiments each: 1–2,036 was significantly less than all other values (p < 0.05); 1,650–2,586, 1,950–2,586 and 2,036–2,586 were all significantly higher than other the other values but were not significantly different from each other (p > 0.05); and 1,072–2,586 and 1,525–2,586 were not significantly different to the Exons value (p > 0.05)