Fig. 2.

Colony-forming unit assay (CFU-f) before transplantation. NA-BMCs were cultivated over 5 days and the number of fibroblastic stem cell colonies derived from these cultures was measured (shown for Balb/c and C57Bl/6 mice). Non-adherent BM cells (NA-BMCs) form CFU-fs over the cultivation period. Representative methylene bluestained cultures from mice total BM cells from day 0–4 (colonies from non-adherent supernatant cells in the first cells that became adherent and proliferated) and shown at ×10 of original magnification (a–e)