Fig. 2.

Recombinant fragments of collagen VII generated in this study. Collagen VII is composed of three identical α chains, each consisting of a central triple helical collagenous domain, flanked by a large amino-terminal noncollagenous domain (NC1) and a smaller carboxy-terminal noncollagenous domain (NC2). Fragments of murine collagen VII cDNA corresponding to the NC1 and NC2 domains were cloned into pGEX-6P-1 and pQE41 and expressed in E. coli. Amino acid residue numbers are shown above the fragments