Immunoblot analysis of the EBA sera reactivity with mCVII. His-tagged recombinant mCVII fragments 1, 2, 3, 4, 5 and Z (lanes 2–8) as well as His-DHFR (lane 1) were separated by 12% SDS-PAGE and electrophoretically transferred to nitrocellulose. The membranes were immunoblotted with a serum from patients EBA1, b EBA3, and c normal human serum (NHS). Migration positions of molecular weight markers (kDa) are shown on the left