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. 2010 Jan 20;67(8):1343–1351. doi: 10.1007/s00018-009-0256-3

Fig. 3.

Fig. 3

Immunoblot analysis of the EBA sera reactivity with mCVII. His-tagged recombinant mCVII fragments 1, 2, 3, 4, 5 and Z (lanes 2–8) as well as His-DHFR (lane 1) were separated by 12% SDS-PAGE and electrophoretically transferred to nitrocellulose. The membranes were immunoblotted with a serum from patients EBA1, b EBA3, and c normal human serum (NHS). Migration positions of molecular weight markers (kDa) are shown on the left