Fig. 5.

EBA patient autoantibodies fix complement to the dermal–epidermal junction of murine skin sections. Frozen sections of human (a,c) and murine (b,d) skin were incubated with serum from a healthy donor (a,b) and from EBA1 patient (c,d). After washing in phosphate-buffered saline, the sections were further incubated with fresh human serum as a source of complement. C3 deposits were visualized by incubation with a monoclonal antibody specific to human C3 followed by an AlexaFluor-488-conjugated anti-mouse IgG antibody. While normal human serum (a,b) did not fix complement in the cryosections, the EBA serum bound complement at the dermal–epidermal junction of both human (c) and murine (d) skin sections