Fig. 6.

EBA patient autoantibodies induce dermal–epidermal separation in sections of murine skin. Frozen sections of human (a,c) and murine (b,d) skin were incubated with serum from a healthy donor (a,b) and from EBA1 patient (c,d). After washing in phosphate-buffered saline, the sections were further incubated with fresh human serum as a source of leucocytes. In contrast to sections incubated with serum from a healthy donor (a,b), sections treated with serum from the EBA patient (c,d) developed dermal–epidermal separation after incubation with normal human granulocytes