Fig. 4.

Effects of ADP receptor ligands on the MAP kinases phosphorylation status of MIN6c4 cells. MIN6c4 cells were incubated for 30 min in medium containing PBS control, 2MeSADP (10 μM) with or without MRS2211 (10 μM). Cell lysates were prepared and then subjected to western blot analysis (20 μg protein/lane), using antibodies against phosphorylated MAPkinases. a,b SAPK/JNK. c,d p44/42. e,f p38. Membranes were reprobed with the anti-GAPDH antibody. Each bar represents the fold increase of phospho-SAPK/JNK, phospho-ERK1/2, or phospho-p38 relative to control after normalizing against total total GAPDH. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3). Data for western blots (b,d,f) are representative for three independent experiments in each group