Fig. 6.

Effects of ADP receptor ligands on the phosphorylation status of Akt/PKB and CREB in MIN6c4 cells. MIN6c4 cells were incubated for 30 min in medium containing: PBS control, 2MeSADP (10 μM) with or without MRS2211 (10 μM). Cell lysates were prepared and then subjected to western blot analysis (20 μg protein/lane), using antibodies against phosphorylated Akt/PKB and ser-133-CREB, respectively. a,b Akt/PKB and c,d ser-133-CREB. Membranes were reprobed with the anti-Akt and CREB antibody. Each bar represents the fold increase of phospho-Akt or phospho-CREB relative to control after normalizing against total non-phosphorylated Akt or CREB. **p < 0.01, ***p < 0.001 (n = 3). Data for western blots (b and d) are representative for three independent experiments in each group