Fig. 7.

Decreased NDRG2 expression attenuates the Akt-mediated anti-apoptosis effect in β-TC3 cells exposed to a high concentration of palmitic acid. a The siRNA sequences used in this experiment. b β-TC3 cells were transfected with 60 nM of three different siRNAs targeting NDRG2 for 48 h, after which samples were collected for Western blotting with antibodies against NDRG2 and α-tubulin. c β-TC3 cells were infected with Ad-myristoylated Aktα for 48 h and transfected with 60 nM NDRG2 siRNA3 or control siRNA and then incubated with 1.0 mM palmitic acid complexed to 1% BSA for 72 h. The apoptotic cells were quantified by flow cytometry. d Western blotting was performed using antibodies against active caspase 3 and β-actin (*P < 0.05 compared to the control)