Fig. 4.

Morphological and functional analyses of mouse embryonic brains in which Sox2 has been conditionally knocked out. a Coronal sections of 19.5 dpc mutant (Sox2^Fl/FL; Nestin-Cre) and littermate control (Sox2^WT/FL; Nestin-Cre) embryos. b Primary, secondary, and tertiary neurosphere assay of 14.5 dpc mutant (Sox2^FL/FL; Nestin-Cre) and littermate control (Sox2^WT/FL) brains. c Attenuated neurogenic potential of Sox2-deficient neurosphere cells. Neurospheres are induced to differentiate using fetal bovine serum and retinoic acid (left panel). Impaired neurogenic potential is also evident in neural stem/progenitor cells cultured as adherent cells. Two panels in a and left panel in c were originally published in [90]. Right panel in c represents original data of S.M. and A.O.