Fig. 7.

The V370G mutant of Kv1.2 was refractory to AHR action. Currents were triggered by serial depolarizing voltages in 10 mV increments from a holding potential of -70 mV in a untransfected H1355 cells, b Kv1.2 WT-expressing H1355 cells, and c Kv1.2 V370G-expressing H1355 cells. d Current densities are plotted against the applied voltages. Results are means ± SEM from 6 to 8 cells of each group. e Currents were triggered by +30 mV depolarization in H1355 cells expressing Kv1.2 WT or V370G channels (upper traces). Currents were recorded after exposure to 50 μM AHR to reach equilibrium (lower traces). f I _eop/I _peak ratio of WT channels in the absence and presence of 50 μM AHR. g I _eop/I _peak ratio of V370G mutant channels in the absence and presence of 50 μM AHR. Results are means ± SEM from 5 to 12 cells of each group. Asterisks indicate statistical significance (p < 0.05)