Fig. 3.
Self-palmitoylation of Bet3 is retarded in mutants that are less efficiently palmitoylated at neutral pH. Purified GST-Bet3 proteins, wild-type, and mutants, as described in Fig. 1, were incubated at pH 7.5 with [3H]-Pal-CoA for 1, 2.5, 5, 7.5, 10, 15, or 30 min at 30°C prior to SDS-PAGE and fluorography. The fluorograms were quantified by densitometry of bands, and relative palmitoylation (absorbance of bands after 30 min of incubation = 100%) is plotted against the time of incubation. Note that individual fluorograms were exposed for different time periods, and, thus, intensity of bands cannot be compared between wild-type and mutant Bet3 proteins